Changes in the gut microbial community of the eastern newt (Notophthalmus viridescens) across its three distinct life stages

Understanding the forces that shape vertebrate gut microbial community assembly and composition throughout development is a major focus of the microbiome field. Here, we utilize the complex life cycle of the eastern newt (Notophthalmus viridescens) as a natural wild model to compare the effects of host and environmental factors on gut microbiome development. We compared bacterial inventories of each of the newt's three physiologically distinct developmental stages to determine if each hosted a unique community, or if the two stages which share an aquatic habitat (larvae and adults) harbored more similar communities than those of the third stage, the terrestrial juvenile eft. Additionally, we assessed how the contribution of selective processes to gut microbial assembly changed through development. We found that structurally, each life stage harbored a distinct community, which may be attributable to host factors. Further, across development, we found that community assembly processes shifted from a predominance of neutral to selective forces. However, habitat may also be important in determining community membership and diversity due the uniqueness of eft communities based on these metrics. Our results are similar to those in other vertebrate taxa, suggesting that gut microbiota assembly processes may be conserved across diverse lineages.

Automated summary

The study focused on understanding the forces shaping vertebrate gut microbial community assembly and composition during different development stages, using the eastern newt as a model. Results showed that each life stage of the newt harbored a distinct gut microbial community, influenced by host factors, and the community assembly processes shifted from neutral to selective forces throughout development. Habitat also played a role in determining community membership and diversity.