Stk24 protects against obesity-associated metabolic disorders by disrupting the NLRP3 inflammasome

Adipose tissue macrophages (ATMs) regulate the occurrence of obesity and its related diseases. Here, we found that serine/threonine protein kinase 24 (Stk24) expression is downregulated significantly in ATMs in obese subjects or obese subjects with type 2 diabetes and mice fed a high-fat diet (HFD). We further identified that glucolipotoxicity downregulated Stk24 expression in ATMs. Stk24-deficient mice develop severe HFD-induced metabolic disorders and insulin insensitivity. Mechanistically, Stk24 intervenes in NLRP3 inflammasome assembly in ATMs by associating directly with NLRP3, decreasing interleukin-1 beta (IL-1 beta) secretion. Accordingly, Stk24 deficiency in the hematopoietic system promotes NLRP3 inflammasome activation, which contributes to exacerbation of metabolic disorders. Intriguingly, Stk24 expression correlates negatively with body mass index (BMI) and the levels of glucose, cholesterol, triglycerides, and low-density lipoprotein in human subjects. These findings provide insights into the function and clinical implications of Stk24 in obesity-mediated metabolic disorders.

Automated summary

In this study, it was found that the expression of Stk24 is significantly downregulated in ATMs in obese individuals or those with type 2 diabetes, as well as in mice fed a high-fat diet. Stk24 deficiency exacerbates HFD-induced metabolic disorders and insulin insensitivity. Stk24 plays a role in regulating NLRP3 inflammasome assembly in ATMs, and its expression correlates negatively with body mass index and related indicators in human subjects.