An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens

In this study, we report a more efficient heterologous expression of lectin from Pleurocybella porrigens (PPL) using an Escherichia coli-based expression system. The yield (9.3 mg/L culture broth) of recombinant PPL (rPPL) using this expression system was increased approximately 9-fold compared to our previous study. The rPPL obtained in this study exhibited the same biochemical properties as the native PPL.

Automated summary

This study successfully increased the expression yield of Pleurocybella porrigens lectin using an Escherichia coli-based expression system, resulting in a 9-fold increase compared to previous studies. The recombinant lectin obtained maintained the same biochemical properties as the native lectin.