期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 85, 期 3, 页码 728-738出版社
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbaa081
关键词
psychrophile-based simple biocatalysts; glycerol dehydratase; 1; 3-propanediol dehydrogenase; aldehyde dehydrogenase; cofactor regeneration system
This study evaluated the simultaneous biosynthesis of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) using a new psychrophile-based simple biocatalyst (PSCat) reaction system. The maximum production of 3-HP was 62.0 mm in solo production and 25.0 mm for 1,3-PDO. Through the PSCat approach, productivity was boosted with a 13.5 mm increase in 3-HP and 4.9 mm increase in 1,3-PDO in the simultaneous production process.
3-Hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) have tremendous potential markets in many industries. This study evaluated the simultaneous biosynthesis of the 2 compounds using the new psychrophile-based simple biocatalyst (PSCat) reaction system. The PSCat method is based on the expression of glycerol dehydratase, 1,3-propanediol dehydrogenase, and aldehyde dehydrogenase from Klebsiella pneumoniae in Shewanella livingstonensis Ac10 and Shewanella frigidimarina DSM 12253, individually. Heat treatment at 45 degrees C for 15 min deactivated the intracellular metabolic flux, and the production process was started after adding substrate, cofactor, and coenzyme. In the solo production process after 1 h, the maximum production of 3-HP was 62.0 m m. For 1,3-PDO, the maximum production was 25.0 m m. In the simultaneous production process, productivity was boosted, and the production of 3-HP and 1,3-PDO increased by 13.5 and 4.9 m m, respectively. Hence, the feasibility of the individual production and the simultaneous biosynthesis system were verified in the new PSCat approach.
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