期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 85, 期 3, 页码 553-561出版社
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbaa040
关键词
autophagy; cell cycle; cyclin D1; gastric carcinoma cells; vitamin C
类别
资金
- Education Department of Shanxi Province under Grant Shanxi 1331 Project Key Innovative Research Team
- Shanxi Province Science Foundation for Youths [201801D221284]
Vitamin C exerts an antiproliferative effect on gastric carcinoma cells by regulating the cell cycle and autophagy through downregulation of Cyclin D1.
Vitamin C has re-emerged as a promising anticancer agent. This study attempts to analyze the differential gene expression of profiles GSE11919 to look for some clues, and the most significant cell cycle pathway caused by vitamin C was identified by integrated bioinformatics analysis. Inspired by this, we investigated the effect of vitamin C treatment on gastric carcinoma cells by detection of cell cycle, apoptosis, and autophagy. Vitamin C significantly elevated the percentage of cells at G0/G1 phase, whereas the percentage of S phase cells was decreased. Meanwhile, vitamin C treatment resulted in downregulation of cell cycle-related protein Cyclin D1. We deduced that the downregulation of Cyclin D1 by vitamin C accompanied by significantly increased 5 ' AMP-activated protein kinase and induced autophagy in MKN45 cells. These results suggest that vitamin C has the antiproliferation effect on gastric carcinoma cells via the regulation of cell cycle and autophagy by Cyclin D1.
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