期刊
NEURO-ONCOLOGY
卷 23, 期 4, 页码 572-585出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/noaa235
关键词
medulloblastoma; lnc-HLX-2-7; MYC; biomarker; therapeutic target
资金
- Ian's Friends Foundation
- The Hough Foundation
- JHU SKCCC [P30 CA006973]
- NCI [5P30CA030199]
- Uehara Memorial Foundation
The novel lncRNA lnc-HLX-2-7 is highly upregulated in Group 3 MB, playing a crucial role in cell proliferation, tumor formation, and apoptosis. It modulates oxidative phosphorylation, mitochondrial dysfunction, and sirtuin signaling pathways in MB. Furthermore, lnc-HLX-2-7 expression is regulated by the MYC oncogene and can be decreased by the small-molecule bromodomain 4 inhibitor JQ1.
Background. Medulloblastoma (MB) is an aggressive brain tumor that predominantly affects children. Recent high-throughput sequencing studies suggest that the noncoding RNA genome, in particular long noncoding RNAs (lncRNAs), contributes to MB subgrouping. Here we report the identification of a novel lncRNA, lnc-HLX-2-7, as a potential molecular marker and therapeutic target in Group 3 MBs. Methods. Publicly available RNA sequencing (RNA-seq) data from 175 MB patients were interrogated to identify lncRNAs that differentiate between MB subgroups. After characterizing a subset of differentially expressed lncRNAs in vitro and in vivo, lnc-HLX-2-7 was deleted by CRISPR/Cas9 in the MB cell line. Intracranial injected tumors were further characterized by bulk and single-cell RNA-seq. Results. Lnc-HLX-2-7 is highly upregulated in Group 3 MB cell lines, patient-derived xenografts, and primary MBs compared with other MB subgroups as assessed by quantitative real-time, RNA-seq, and RNA fluorescence in situ hybridization. Depletion of lnc-HLX-2-7 significantly reduced cell proliferation and 3D colony formation and induced apoptosis. Lnc-HLX-2-7-deleted cells injected into mouse cerebellums produced smaller tumors than those derived from parental cells. Pathway analysis revealed that lnc-HLX-2-7 modulated oxidative phosphorylation, mitochondrial dysfunction, and sirtuin signaling pathways. The MYC oncogene regulated lnc-HLX-2-7, and the small-molecule bromodomain and extraterminal domain family.bromodomain 4 inhibitor Jun Qi 1 (JQ1) reduced lnc-HLX-2-7 expression. Conclusions. Lnc-HLX-2-7 is oncogenic in MB and represents a promising novel molecular marker and a potential therapeutic target in Group 3 MBs.
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