3.8 Article

Analysis of Salivary Metabolites by Nuclear Magnetic Resonance Before and After Oral Mucosa Cleaning of Infants in the Pre-dental Period

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FRONTIERS IN DENTAL MEDICINE
卷 2, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fdmed.2021.667365

关键词

saliva; magnetic resonance spectroscopy; infant; oral hygiene; breast feeding; metabolomics

资金

  1. FAPERJ
  2. CNPq
  3. CAPES [001]
  4. National Institute of Science and Technology of Structural Biology and Bioimaging (INCT-INBEB)

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This study found that cleaning the oral cavity of infants in the pre-dental period can reduce the concentration of extrinsic components such as lactose in salivary metabolomic profile. Infants who were exclusively breastfed had higher levels of lactose in their saliva compared to those who received mixed feeding.
The aim of the present study was to verify if a protocol for cleaning the oral cavity of infants in the pre-dental period can reduce extrinsic salivary metabolites observed through Nuclear Magnetic Resonance (NMR). A cross-sectional clinical study with a convenience sample was conducted, and infants were recruited at the UFRJ Pediatric Dentistry Clinic. Participants who had used antibiotics and/or antifungals up to 3 months before and whose legal guardians did not consent or sign the Informed Consent Form were excluded. An anamnesis was performed with the guardians and the participants' intraoral clinical examination. Initial collection of unstimulated total saliva was performed using an automatic pipette with sterile plastic tips in the buccal floor region, at least 1 h after the last feeding. Subsequently, the infants' oral mucosa was cleaned with gauze moistened with filtered water, and after 5 min, a new collection was performed, using the same methodology. The obtained samples were immediately transferred on ice to the laboratory, centrifuged (10,000 g), and stored at -80(?)C. The NMR analyses were performed using a 500-MHz spectrometer Bruker, Germany); evaluations were done via the H-1 and H-1 -H-1 TOCSY spectra for metabolite signaling. Eleven pre-dental infants were evaluated, with a mean age of 3.8 months, including six girls (55%). Of these, nine participants (82%) were exclusively breastfed. The higher presence of components such as lactose, glucose, sugars, acetate, alanine, and lactate were observed in the samples before oral mucosa cleaning. Regarding the type of diet, more lactose was observed in the saliva of patients who were exclusively breastfed than those that received mixed feeding. We conclude that the oral mucosa cleaning of infants in the pre-dental period tends to reduce the concentration of extrinsic components from the diet, such as lactose, in the salivary metabolomic profile analyzed by NMR.

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