期刊
FEBS LETTERS
卷 591, 期 5, 页码 792-800出版社
WILEY
DOI: 10.1002/1873-3468.12593
关键词
apocarotenoid; carlactone; carotenoid; carotenoid cleavage dioxygenase; strigolactone
资金
- King Abdullah University of Science and Technology (KAUST)
Strigolactones are a new class of phytohormones synthesized from carotenoids via carlactone. The complex structure of carlactone is not easily deducible from its precursor, a cis-configured beta-carotene cleavage product, and is thus formed via a poorly understood series of reactions and molecular rear-rangements, all catalyzed by only one enzyme, the carotenoid cleavage dioxygenase 8 (CCD8). Moreover, the reactions leading to carlactone are expected to form a second, yet unidentified product. In this study, we used C-13 and O-18-labeling to shed light on the reactions catalyzed by CCD8. The characterization of the resulting carlactone by LC-MS and NMR, and the identification of the assumed, less accessible second product allowed us to formulate a minimal reaction mechanism for carlactone generation.
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