期刊
FEBS LETTERS
卷 591, 期 5, 页码 706-717出版社
WILEY
DOI: 10.1002/1873-3468.12574
关键词
antimicrobial peptide; electrostatics calculations; molecular dynamics; nucleic acid
资金
- National Institute of Allergy and Infectious Diseases (NIH-NIAID) Award [R15AI079685]
- National Science Foundation (NSF) Award [MCB1615313, CHE-1005032]
- Extreme Science and Engineering Discovery Environment (XSEDE) allocation [MCB120100]
- Arnold Beckman Scholars Program
While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Here, molecular modeling and experimental measurements are used to show that neither nucleic acid binding peptide selectively binds DNA sequences. Simulations and experiments also show that changing lysines to arginines enhances DNA binding, suggesting that including additional guanidinium groups is a potential strategy to engineer more potent AMPs. Moreover, the lack of binding specificity may make it more difficult for bacteria to evolve resistance to these and other similar AMPs.
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