期刊
FEBS LETTERS
卷 591, 期 8, 页码 1073-1082出版社
WILEY
DOI: 10.1002/1873-3468.12529
关键词
genetic recombination
资金
- Biotechnology and Biological Sciences Research Council [B17092, BB/P001491/1, BB/E001777/1] Funding Source: researchfish
- Cancer Research UK [11722, 18604] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J017094/1] Funding Source: researchfish
- BBSRC [BB/E001777/1, BB/P001491/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E001777/1, B17092] Funding Source: Medline
- Cancer Research UK [11722] Funding Source: Medline
Holliday junction-resolving enzymes are nucleases that are highly specific for the structure of the junction, to which they bind in dimeric form. Two symmetrically disposed cleavages are made. These are not simultaneous, but the second cleavage is accelerated relative to the first, so ensuring that bilateral cleavage occurs during the lifetime of the DNA-protein complex. In eukaryotic cells there are two known junction-resolving activities. GEN1 is similar to enzymes from lower organisms. A crystallographic structure of a fungal GEN1 bound to the product of resolution has been determined. These complexes are dimerized within the crystal lattice such that the strands of the products may be simply reconnected to form a junction. These structures suggest a trajectory for the resolution process.
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