期刊
SCIENCE ADVANCES
卷 7, 期 22, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abf9000
关键词
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资金
- National Natural Science Foundation of China [22072159]
- Fundamental Research Funds for the Central Universities [buctrc202015]
Cells are compartmentalized microreactors with molecularly crowded microenvironments, inspiring the design of synthetic analogs to mimic their functionality. The integration of membraneless coacervate microdroplets into a proteinosome creates hierarchical protocells that can sense extracellular signals and accelerate enzymatic reactions. These protocells are also capable of signal processing for designing Boolean logic gates and provide a flexible synthetic platform for studying complex metabolic reaction networks and chemical computation.
As the basic unit of life, cells are compartmentalized microreactors with molecularly crowded microenvironments. The quest to understand the cell origin inspires the design of synthetic analogs to mimic their functionality and structural complexity. In this work, we integrate membraneless coacervate microdroplets, a prototype of artificial organelles, into a proteinosome to build hierarchical protocells that may serve as a more realistic model of cellular organization. The protocell subcompartments can sense extracellular signals, take actions in response to these stimuli, and adapt their physicochemical behaviors. The tiered protocells are also capable of enriching biomolecular reactants within the confined organelles, thereby accelerating enzymatic reactions. The ability of signal processing inside protocells allows us to design the Boolean logic gates (NOR and NAND) using biochemical inputs. Our results highlight possible exploration of protocell--community signaling and render a flexible synthetic platform to study complex metabolic reaction networks and embodied chemical computation.
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