4.8 Article

SlERF52 regulates SlTIP1;1 expression to accelerate tomato pedicel abscission

期刊

PLANT PHYSIOLOGY
卷 185, 期 4, 页码 1829-1846

出版社

OXFORD UNIV PRESS INC
DOI: 10.1093/plphys/kiab026

关键词

-

资金

  1. National Key Research and Development Program of China [2018YFD1000800]
  2. National Natural Science Foundation of China [31572167, 31672197, U1708232, 31861143045]
  3. Liaoning Revitalization talent program [2018050]
  4. China Scholarship Council

向作者/读者索取更多资源

Abscission of plant organs is controlled by various signals and environmental stimuli, involving multiple regulatory networks like auxin flux and ethylene production. Tonoplast intrinsic proteins play a crucial role in abscission by regulating H2O2 levels and water permeability. These proteins interact with cytoplasmic H2O2 and water influx to modulate the abscission process.
Abscission of plant organs is induced by developmental signals and diverse environmental stimuli and involves multiple regulatory networks, including biotic or abiotic stress-impaired auxin flux in the abscission zone (AZ). Depletion of auxin activates AZ ethylene (ETH) production and triggers acceleration of abscission, a process that requires hydrogen peroxide (H2O2). However, the interaction between these networks and the underlying mechanisms that control abscission are poorly understood. Here, we found that expression of tonoplast intrinsic proteins, which belong to the aquaporin (AQP) family in the AZ was important for tomato (Solanum lycopersicum) pedicel abscission. Liquid chromatography-tandem mass spectrometry and in situ hybridization revealed that SlTIP1;1 was most abundant and specifically present in the tomato pedicel AZ. SlTIP1;1 localized in the plasma membrane and tonoplast. Knockout of SlTIP1;1 resulted in delayed abscission, whereas overexpression of SlTIP1;1 accelerated abscission. Further analysis indicated that SlTIP1;1 mediated abscission via gating of cytoplasmic H2O2 concentrations and osmotic water permeability (P-f). Elevated cytoplasmic levels of H2O2 caused a suppressed auxin signal in the early abscission stage and enhanced ETH production during abscission. Furthermore, we found that increasing Pf was required to enhance the turgor pressure to supply the break force for AZ cell separation. Moreover, we observed that SlERF52 bound directly to the SlTIP1;1 promoter to regulate its expression, demonstrating a positive loop in which cytoplasmic H2O2 activates ETH production, which activates SlERF52. This, in turn, induces SlTIP1;1, which leads to elevated cytoplasmic H2O2 and water influx.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据