期刊
PLANT CELL
卷 33, 期 2, 页码 306-321出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/plcell/koaa018
关键词
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资金
- National Key Research and Development Program of China [2016YFD0101007]
- National Natural Science Foundation of China [31530066, 31322047, 31701933]
- Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Science (ASTIP-CAAS) [CAAS-XTCX2016001]
- Leading Talents of Guangdong Province Program [00201515]
- Shenzhen municipal (The Peacock Plan) [KQTD2016113010482651]
- Dapeng district governments, Central Public-interest Scientific Institution Basal Research Fund [Y2017PT52]
- Taishan Scholar Foundation of the People's Government of Shandong Province
Unisexual flowers in cucumber are controlled by three major loci, including the F locus which determines female development. ACS1G is responsible for conferring gynoecy in the F locus, and its early expression with ACO2 leads to ethylene production, repressing WIP1 and activating ACS2 to initiate female floral development. This cooperative mechanism involving ethylene biosynthesis genes establishes a dominant pathway for controlling unisexual flower development in cucumber.
Unisexual flowers provide a useful system for studying plant sex determination. In cucumber (Cucumis sativus L.), three major Mendelian loci control unisexual flower development, Female (F), androecious [a; 1-aminocyclopropane-1-carboxylate {ACC} synthase 11, acs11], and Monoecious (M; ACS2), referred to here as the Female, Androecious, Monoecious (FAM) model, in combination with two genes, gynoecious (g, the WIP family C2H2 zinc finger transcription factor gene WIP1) and the ethylene biosynthetic gene ACC oxidase 2 (ACO2). The F locus, conferring gynoecy and the potential for increasing fruit yield, is defined by a 30.2-kb tandem duplication containing three genes. However, the gene that determines the Female phenotype, and its mechanism, remains unknown. Here, we created a set of mutants and revealed that ACS1G is responsible for gynoecy conferred by the F locus. The duplication resulted in ACS1G acquiring a new promoter and expression pattern; in plants carrying the F locus duplication, ACS1G is expressed early in floral bud development, where it functions with ACO2 to generate an ethylene burst. The resulting ethylene represses WIP1 and activates ACS2 to initiate gynoecy. This early ACS1G expression bypasses the need for ACS11 to produce ethylene, thereby establishing a dominant pathway for female floral development. Based on these findings, we propose a model for how these ethylene biosynthesis genes cooperate to control unisexual flower development in cucumber.
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