Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2

Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is an emerging need of the current pandemic. Imaging at near infrared second biological window (NIR-II) offers striking benefits over the other technologies to explore deep-tissue information. Here we design, synthesise and characterise a molecular probe that selectively targets the N-gene of SARS-CoV-2. Highly specific antisense oligonucleotides (ASOs) were conjugated to lead sulfide quantum dots using a UV-triggered thiol-ene click chemistry for the recognition of viral RNA. Our ex vivo imaging studies demonstrated that the probe exhibits aggregation induced NIR-II emission only in presence of SARS-CoV-2 RNA which can be attributed to the efficient hybridisation of the ASOs with their target RNA strands.

Automated summary

Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is crucial, and NIR-II imaging technology shows promising benefits in exploring deep-tissue information. A molecular probe designed in this study selectively targets the N-gene of SARS-CoV-2 using specific antisense oligonucleotides conjugated to lead sulfide quantum dots. Ex vivo imaging studies demonstrated that the probe emits NIR-II only in the presence of SARS-CoV-2 RNA, indicating efficient hybridization of the ASOs with their target RNA strands.