4.6 Article

NLP1 reciprocally regulates nitrate inhibition of nodulation through SUNN-CRA2 signaling in Medicago truncatula

期刊

PLANT COMMUNICATIONS
卷 2, 期 3, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.xplc.2021.100183

关键词

CRA2; CEP; CLE; nitrate inhibition of nodulation; NLP1; SUNN

资金

  1. National Key Research & Development Program of China [2016YFA0500500, 2016YFD0100702]
  2. NSFC [31670242]

向作者/读者索取更多资源

Most legume plants form nodules with rhizobia, regulated by signaling pathways involving CLE/SUNN and CEP/CRA2. Nitrate inhibits nodulation through local and systemic pathways, with NLP1 playing a key role. Further research is needed to understand the complex interactions between these pathways in mediating nodulation inhibition by nitrate.
Most legume plants can associate with diazotrophic soil bacteria called rhizobia, resulting in new root organs called nodules that enable N-2 fixation. Nodulation is an energy-consuming process, and nodule number is tightly regulated by independent systemic signaling pathways controlled by CLE/SUNN and CEP/CRA2. Moreover, nitrate inhibits legume nodulation via local and systemic regulatory pathways. In Medicago truncatula, NLP1 plays important roles in nitrate-induced inhibition of nodulation, but the relationship between systemic and local pathways in mediating nodulation inhibition by nitrate is poorly understood. In this study, we found that nitrate induces CLE35 expression in an NLP1-dependent manner and that NLP1 binds directly to the CLE35 promoter to activate its expression. Grafting experiments revealed that the systemic control of nodule number involves negative regulation by SUNN and positive regulation by CRA2 in the shoot, and that NLP1's control of the inhibition of rhizobial infection, nodule development, and nitrogenase activity in response to nitrate is determined by the root. Unexpectedly, grafting experiments showed that loss of CRA2 in the root increases nodule number at inhibitory nitrate levels, probably because of CEP1/2 upregulation in the cra2 mutants, suggesting that CRA2 exerts active negative feedback regulation in the root.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据