Synchrotron micro-X-ray fluorescence imaging of arsenic in frozen-hydrated sections of a root of Pteris vittata

We performed micro-X-ray fluorescence imaging of frozen-hydrated sections of a root of Pteris vittata for the first time, to the best of our knowledge, to reveal the mechanism of arsenic (As) uptake. The As distribution was successfully visualized in cross sections of different parts of the root, which showed that (i) the major pathway of As uptake changes from symplastic to apoplastic transport in the direction of root growth, and (ii) As and K have different mobilities around the stele before xylem loading, despite their similar distributions outside the stele in the cross sections. These data can reasonably explain As reduction, axially observed around the root tip in the direction of root growth and radially observed in the endodermis in the cross sections, as a consequence of the incorporation of As into the cells or symplast of the root. In addition, previous observations of As species in the midrib can be reconciled by ascribing a reduction capacity to the root cells, which implies that As reduction mechanisms at the cellular level may be an important control on the peculiar root-to-shoot transport of As in P. vittata.

Automated summary

Micro-X-ray fluorescence imaging was used for the first time to study the mechanism of arsenic uptake in frozen-hydrated sections of a Pteris vittata root. The distribution of arsenic was visualized, showing changes in uptake pathways and differing mobilities of arsenic and potassium in different parts of the root. These findings provide insights into the peculiar root-to-shoot transport of arsenic in P. vittata.