期刊
NATURE PLANTS
卷 7, 期 6, 页码 842-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41477-021-00932-y
关键词
-
资金
- National Science Foundation (RESEARCH-PGR grant) [1748843]
- German Research Foundation (DFG) [441540116]
- National Institutes of Health (T32 training grant) [HG000035, R01-GM079712]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1748843] Funding Source: National Science Foundation
A massively parallel reporter assay was used to measure the activity of nearly complete sets of promoters from Arabidopsis, maize and sorghum in two assay systems, uncovering the sequence features affecting promoter strength and facilitating promoter strength prediction and synthetic design. The experiments revealed species-specific differences in the contributions of GC content and transcription factors to promoter strength.
Targeted engineering of plant gene expression holds great promise for ensuring food security and for producing biopharmaceuticals in plants. However, this engineering requires thorough knowledge of cis-regulatory elements to precisely control either endogenous or introduced genes. To generate this knowledge, we used a massively parallel reporter assay to measure the activity of nearly complete sets of promoters from Arabidopsis, maize and sorghum. We demonstrate that core promoter elements-notably the TATA box-as well as promoter GC content and promoter-proximal transcription factor binding sites influence promoter strength. By performing the experiments in two assay systems, leaves of the dicot tobacco and protoplasts of the monocot maize, we detect species-specific differences in the contributions of GC content and transcription factors to promoter strength. Using these observations, we built computational models to predict promoter strength in both assay systems, allowing us to design highly active promoters comparable in activity to the viral 35S minimal promoter. Our results establish a promising experimental approach to optimize native promoter elements and generate synthetic ones with desirable features. A massively parallel reporter assay was used to measure the activity of nearly complete sets of promoters from Arabidopsis, maize and sorghum in two assay systems, uncovering the sequence features affecting promoter strength and facilitating promoter strength prediction and synthetic design.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据