期刊
AMERICAN JOURNAL OF CANCER RESEARCH
卷 11, 期 5, 页码 2081-2094出版社
E-CENTURY PUBLISHING CORP
关键词
CDK1; laryngeal squamous cell carcinoma (LSCC); gene silencing; cell cycle checkpoint
类别
资金
- Polish National Science Center [2013/11/N/NZ2/02483]
CDK1 is essential for cell proliferation and cycle regulation, making it a potential target in cancer therapy. Knockdown of CDK1 in LSCC cell lines did not impact cell proliferation and viability, with analysis showing enriched pathways in differentially expressed genes. Some genes, such as CDK6, CALD1, and FYN, are potentially dependent on CDK1.
Alterations of the cell cycle checkpoints lead to uncontrolled cell growth and result in tumorigenesis. One of the genes essential for cell proliferation and cell cycle regulation is CDK1. This makes it a potential target in cancer therapy. In our previous study we have shown upregulation of this gene in laryngeal squamous cell carcinoma (LSCC). Here we analyze the impact of siRNA-mediated CDK1 knockdown on cell proliferation and viability, measured with cell growth monitoring and colorimetric test (CCK8 assay), respectively. We proved that a reduction of CDK1 expression by more than 50% has no effect on these cellular processes in LSCC cell lines (n=2). Moreover, using microarrays, we analyzed global gene expression deregulation in these cell lines after CDK1 knockdown. We searched for enriched ontologies in the group of identified 137 differentially expressed genes (>2-fold change). Within this group we found 3 enriched pathways: protein binding (GO:0005515), mitotic nuclear division (GO:0007067) and transmembrane receptor protein tyrosine kinase signaling pathway (GO:0007169) and a group of 11 genes encoding proteins for which interaction with CDK1 was indicated with the use of bioinformatic tools. Among these genes we propose three: CDK6, CALD1 and FYN as potentially dependent on CDK1.
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