期刊
FOOD & FUNCTION
卷 12, 期 14, 页码 6374-6388出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1fo00461a
关键词
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资金
- Key Project of the Education Department of Hunan Province [20A529]
- project 2011 Collaborative Innovation Center of Hunan Province [448]
delta-Tocotrienol demonstrates chemopreventive effects on nasopharyngeal carcinoma cells by inducing apoptosis and cell cycle arrest. Gene expression analysis reveals its impact on CNE1 cells' gene regulation.
Nasopharyngeal carcinoma has a notably high incidence rate in Southern China, Southeast Asia, North Africa, Middle East, and the Arctic. delta-Tocotrienol is abundant in cereal and has some health benefits. In our recent study, we showed that delta-tocotrienol exerted anti-inflammatory effects in murine macrophages in vitro. The aim of this study was to further investigate the chemopreventive effects of delta-tocotrienol on human CNE1 cells. We showed that delta-tocotrienol induced apoptosis and cell cycle arrest at G(0)/G(1) and M phases in nasopharyngeal carcinoma cells. Microarray analysis revealed that after CNE1 cells were treated with delta-tocotrienol, 169 genes were up-regulated and 167 down-regulated. ERK1/2 was shown to play a vital role in cell cycle arrest by gene chips. The results suggest that delta-tocotrienol induces cell cycle arrest in CNE1 cells via the p16/CDK4/cyclin D1 signaling pathway. Western blots showed that CNE1 apoptosis was related to dysregulated expression of Bax-2 and Bcl-2. Furthermore, caspase-3, -8, -9 up-regulation was related to the apoptotic effect of delta-tocotrienol; therefore, delta-tocotrienol triggers apoptosis in CNE1 cells through caspase-3 signaling. delta-Tocotrienol may potentially be developed as an anti-cancer agent in the management of nasopharyngeal carcinoma.
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