期刊
JOURNAL OF CANCER
卷 12, 期 13, 页码 3967-3975出版社
IVYSPRING INT PUBL
DOI: 10.7150/jca.45493
关键词
Demethylzeylasteral; T-96; ESCC; proliferation; cell cycle; apoptosis; EMT; Wnt/beta-catenin pathway
类别
资金
- Young Top-Notch talent Project of Hebei province [JI2016(10)]
- Talent Project of Hebei province [A201801005]
- Academician Workstation Construction Special Project Of Tangshan People's Hospital [199A77119H]
- Natural Science Foundation of Outstanding Youth of Hebei Province [H2019105026]
- Basic Research Cooperation Project of Beijing-Tianjin-Hebei [H2019105143, 19JCZDJC64500(Z)]
T-96 inhibits the proliferation and migration of esophageal cancer cells through Wnt/beta-catenin pathway, leading to cell cycle arrest and apoptosis, providing a potential treatment for esophageal squamous cell carcinoma.
As a kind of tumor commonly seen, no effective treatment is available for esophageal squamous cell carcinoma (ESCC). Therefore, seeking a new treatment is urgent. Demethylzeylasteral (T-96) isolated from Tripterygium wilfordii root bark embraces outstanding good antitumor activity. However, as for the mechanism of T-96 work on ESCC cells, it is rarely reported. In this study, we found that T-96 has inhibition when ESCC cells are proliferating, migrating and cloning. Moreover, relevant effects are influenced by dose and time. And T-96 can result in the stop of G2/M phase and induce apoptosis of ESCC cells. In addition, the expressions of Cyclin B1, Cyclin D1, Bcl-2, PARP1 and Survivin were decreased after starch demethylation. Despite of this, Bax and PARP1's expressions went up. To add up, there was an obvious increase in the expression of E-cadherin, while that of N-cadherin, Vimentin and MMP9 decreased after T-96 treatment. Moreover, the expression of Wnt/beta-Catenin pathway, which concerns proteins beta-Catenin, c-Myc and Wnt3a decreased. Our study shows that T-96 inhibits the proliferation and migration of esophageal cancer cells through Wnt/beta-catenin pathway. Moreover, it gives rise to cell cycle arrest and apoptosis. According to the research results, T-96 tends to be put into use when treating ESCC patients, thus laying the experimental foundation for clinical research.
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