期刊
MOLECULAR BIOLOGY REPORTS
卷 48, 期 5, 页码 4413-4420出版社
SPRINGER
DOI: 10.1007/s11033-021-06459-x
关键词
Recurrent pregnancy loss; Progesterone receptor gene; Estrogen receptor 1 gene; Polymorphisms; PCR-RFLP; DNA sequencing
This study investigated the association of SNPs of ESR1 and PR genes with RPL in an Egyptian population, showing higher significance of ESR1 gene SNPs (rs2234693) and (rs9340799) in RPL cases compared to controls, as well as significantly increased frequencies of PR gene variants S344T (rs3740753) and H770H (rs1042839) in women. A new P352Q polymorphism was also observed. There is a significant association of ESR1 and PR gene SNPs with RPL, warranting further research to verify these findings.
Recurrent pregnancy loss (RPL) represents one of the pregnancy complications affecting 1-3% of women. Sex hormones, progesterone and estrogen play a critical role in the maintenance of pregnancy; they are mediated by estrogen receptor 1 (ESR1) and progesterone receptor (PR) genes respectively. Polymorphisms of (ESR1) and (PR) genes are linked to RPL. We aimed to explore the association of single nucleotide polymorphisms (SNPs) of (ESR1) gene and (PR) gene with RPL in a cohort of Egyptian population (50 infertile Egyptian women who experienced RPL and 50 healthy women), using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) of (ESR1) gene and DNA sequencing of exons 1 and 5 of (PR) gene. Genotyping of ESR1 gene SNP's: (rs2234693) and (rs9340799) revealed higher significance in cases compared to controls of p value (p = 0.006 and p = 0.001) respectively. However, the frequencies of the two variants in (PG) gene; S344T (rs3740753) (p = 0.0001) and H770H (rs1042839) (p = 0.001) were significantly higher in women compared to the healthy control women. New polymorphism P352Q was observed in 2% of cases (p = 0.0001). There was a significant association of SNP's of ESR1 and PR genes with recurrent pregnancy loss RPL. Further demographics studies should be carried on a larger number of women at risk of recurrent implantation to elucidate this SNP's association and its role in RPL women.
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