期刊
JOURNAL OF THORACIC DISEASE
卷 13, 期 5, 页码 3115-3125出版社
AME PUBL CO
DOI: 10.21037/jtd-21-752
关键词
Squamous cell lung carcinoma; squamous cell lung cancer (SqCLC); discs large homolog 5 (Dlg5); epithelial-mesenchymal transition (EMT); E-cadherin (E-cad)
资金
- Research and Cultivation Fund of Capital Medical University [FYZ2017148]
- Beijing Natural Science Foundation [7184200]
Dlg5 expression is significantly correlated with lymph node metastasis and disease recurrence in squamous cell lung cancer patients, with low expression associated with poor outcomes. Dlg5 may have potential predictive value for overall survival in SqCLC patients.
Background: Discs large homolog 5 (Dlg5) is a newly discovered member of the membrane-associated guanylate kinase superfamily (MAGUK) that is involved in several important processes, including the maintenance of epithelial cell polarity, cell proliferation control, and cell migration and invasion. Decreased expression of Dlg5 has been reported in malignancies arising from different organs. In the present study, we analyzed Dlg5 expression and its prognostic value in squamous cell lung cancer (SqCLC). Methods: Tumor tissue and adjacent normal tissue sections were collected from 98 patients with SqCLC. The expression levels of Dlg5 and epithelial-mesenchymal transition (EMT) biomarkers in the tissue sections were examined by immunohistochemistry and western blot. Results: There were 80 males and 18 females in the study cohort. Patients at pathological stages I and IIIA accounted for 64.3% and 35.7% of the cohort, respectively. Western blot showed that Dlg5 expression differed between SqCLC and healthy tissues. Western blot also revealed low Dlg5 expression to be associated with low E-cadherin expression and high vimentin expression, which was consistent with the findings of immunohistochemical staining. Dlg5 expression was significantly correlated with lymph node (LN) metastasis (P=0.001) and disease recurrence (P<0.001), as well as with E-cadherin and vimentin expression (P=0.025 and P=0.001, respectively). Univariate analysis showed that overall survival was significantly correlated with the tumor-node-metastasis (P<0.001) and T (P=0.001) stages, LN metastasis (P<0.001), Dig5 expression (P<0.001), 0-catenin expression (P=0.004), and vimentin expression (P=0.002). Patients with overexpression of Dlg5 and 0-catenin had a more favorable prognosis than those without. Multivariate analysis revealed that tumor-node-metastasis stage [hazard ratio (HR) =2.124; 95% confidence interval (CI), 1.195-3.777; P=0.010], Dlg5 expression (HR =0.548; 95% CI, 0.313-0.959; P=0.035), 0-catenin expression (HR =0.545; 95% CI, 0.312-0.953; P=0.033), and vimentin expression (HR =1.850; 95% CI, 1.050-3.258; P=0.033) could all independently predict the overall survival of patients with SqCLC. Conclusions: Dlg5 is an important player in EMT which may have potential predictive value for SqCLC prognosis after surgery. Background: Discs large homolog 5 (Dlg5) is a newly discovered member of the membrane-associated guanylate kinase superfamily (MAGUK) that is involved in several important processes, including the maintenance of epithelial cell polarity, cell proliferation control, and cell migration and invasion. Decreased expression of Dlg5 has been reported in malignancies arising from different organs. In the present study, we analyzed Dlg5 expression and its prognostic value in squamous cell lung cancer (SqCLC). Methods: Tumor tissue and adjacent normal tissue sections were collected from 98 patients with SqCLC. The expression levels of Dlg5 and epithelial-mesenchymal transition (EMT) biomarkers in the tissue sections were examined by immunohistochemistry and western blot. Results: There were 80 males and 18 females in the study cohort. Patients at pathological stages I and IIIA accounted for 64.3% and 35.7% of the cohort, respectively. Western blot showed that Dlg5 expression differed between SqCLC and healthy tissues. Western blot also revealed low Dlg5 expression to be associated with low E-cadherin expression and high vimentin expression, which was consistent with the findings of immunohistochemical staining. Dlg5 expression was significantly correlated with lymph node (LN) metastasis (P=0.001) and disease recurrence (P<0.001), as well as with E-cadherin and vimentin expression (P=0.025 and P=0.001, respectively). Univariate analysis showed that overall survival was significantly correlated with the tumor-node-metastasis (P<0.001) and T (P=0.001) stages, LN metastasis (P<0.001), Dig5 expression (P<0.001), 0-catenin expression (P=0.004), and vimentin expression (P=0.002). Patients with overexpression of Dlg5 and 0-catenin had a more favorable prognosis than those without. Multivariate analysis revealed that tumor-node-metastasis stage [hazard ratio (HR) =2.124; 95% confidence interval (CI), 1.195-3.777; P=0.010], Dlg5 expression (HR =0.548; 95% CI, 0.313-0.959; P=0.035), 0-catenin expression (HR =0.545; 95% CI, 0.312-0.953; P=0.033), and vimentin expression (HR =1.850; 95% CI, 1.050-3.258;
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据