期刊
EXPERT REVIEW OF PROTEOMICS
卷 14, 期 5, 页码 419-429出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14789450.2017.1322904
关键词
Data-independent acquisition; SWATH; mass spectrometry; quantification; proteomics; clinic; biomarker; SRM; PRM
资金
- National Institute of Diabetes and Digestive and Kidney Diseases [R24DK085610]
- NIH T32 [T32G000266]
- NIH [1S10 OD016281]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R24DK085610] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [T32AG000266] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [S10OD016281] Funding Source: NIH RePORTER
Introduction: While selected/multiple-reaction monitoring (SRM or MRM) is considered the gold standard for quantitative protein measurement, emerging data-independent acquisition (DIA) using high-resolution scans have opened a new dimension of high-throughput, comprehensive quantitative proteomics. These newer methodologies are particularly well suited for discovery of biomarker candidates from human disease samples, and for investigating and understanding human disease pathways.Areas covered: This article reviews the current state of targeted and untargeted DIA mass spectrometry-based proteomic workflows, including SRM, parallel-reaction monitoring (PRM) and untargeted DIA (e.g., SWATH). Corresponding bioinformatics strategies, as well as application in biological and clinical studies are presented.Expert commentary: Nascent application of highly-multiplexed untargeted DIA, such as SWATH, for accurate protein quantification from clinically relevant and disease-related samples shows great potential to comprehensively investigate biomarker candidates and understand disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据