期刊
ACS APPLIED BIO MATERIALS
卷 4, 期 5, 页码 4432-4440出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.1c00188
关键词
poly(4-hydroxybutyrate) (P4HB); double-emulsion solvent evaporation method; open porous microsphere; bone tissue engineering; bone defect
资金
- Shandong Provincial Natural Science Foundation, China [ZR2017BEM019, 2015GGX103037]
- National Natural Science Foundation of China [51803106, 22031005]
- National Key Research and Development Program of China [2016YFB1100805]
This study demonstrates that injectable open porous P4HB microspheres have significant potential in bone tissue regeneration by enhancing cell proliferation and accelerating bone defect healing.
Delivering injectable microspheres in a minimally invasive way to repair complexly shaped tissue defects renders them attractive for clinical use. Especially, open porous microspheres that provide sufficient internal space for cell proliferation and nutrient diffusions can efficiently aid to completing reconstructions of tissue defects. In this work, chemically synthesized and biodegradable poly(4-hydroxybutyrate) (P4HB), which is the U.S. FDA-approved polyhydroxyalkanoate (PHA), was employed for fabricating open porous microspheres using a double-emulsion solvent evaporation method. The influences of fabrication parameters were discussed. It was found that the P4HB-based cell-free and growth factor-free open porous microspheres can enhance osteoblast differentiation of adipose-derived stem cells in vitro and accelerate rat calvarial bone-defect healing in vivo. These results demonstrated that the injectable open porous P4HB microspheres present a remarkable potential in bone tissue regeneration.
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