期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 9, 期 24, 页码 4863-4872出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1tb00775k
关键词
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资金
- COST Action [AC15209]
- European Union Horizon 2020 research and innovation program [668119]
- ATTRACT project - EC [777222]
This study focuses on developing innovative sensors to monitor tissue implant status non-invasively by using Fast-Field-Cycling Magnetic Resonance Imaging (FFC-MRI). These sensors, based on oligo-histidine moieties, can generate a specific quadrupolar relaxation enhancement at 1.39 MHz, serving as an indicator of scaffold integrity and exhibiting good biocompatibility.
This study is focused on the development of innovative sensors to non-invasively monitor the tissue implant status by Fast-Field-Cycling Magnetic Resonance Imaging (FFC-MRI). These sensors are based on oligo-histidine moieties that are conjugated to PLGA polymers representing the structural matrix for cells hosting scaffolds. The presence of N-14 atoms of histidine causes a quadrupolar relaxation enhancement (also called Quadrupolar Peak, QP) at 1.39 MHz. This QP falls at a frequency well distinct from the QPs generated by endogenous semisolid proteins. The relaxation enhancement is pH dependent in the range 6.5-7.5, thus it acts as a reporter of the scaffold integrity as it progressively degrades upon lowering the microenvironmental pH. The ability of this new sensors to generate contrast in an image obtained at 1.39 MHz on a FFC-MRI scanner is assessed. A good biocompatibility of the histidine-containing scaffolds is observed after its surgical implantation in healthy mice. Over time the scaffold is colonized by endogenous fibroblasts and this process is accompanied by a progressive decrease of the intensity of the relaxation peak. In respect to the clinically used contrast agents this material has the advantage of generating contrast without the use of potentially toxic paramagnetic metal ions.
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