期刊
BMC ECOLOGY AND EVOLUTION
卷 21, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12862-021-01825-6
关键词
Testis; Genes; Phylogeny; Hymenoptera
资金
- Centre regional government (FERTILMALE) [2017 119977]
- French National Research Agency (ANR) (FertiliWasp project) [ANR-14-CE21-0004-01]
- INRAE
- University of Tours
- FertiliWasp
- FERTILMALE
- Agence Nationale de la Recherche (ANR) [ANR-14-CE21-0004] Funding Source: Agence Nationale de la Recherche (ANR)
This study identified candidate genes that may play a significant role in spermatogenesis in hymenopterans by comparing taxonomic, phenotypic data, and gene expression. The research found that a large percentage of genes expressed in mice and fruit flies also had orthologs in hymenopteran species, suggesting their importance in testis function in insects. Furthermore, a majority of genes involved in male fertility in Drosophila were found to have orthologs in Hymenoptera species, indicating potential conservation of these genes across different insect taxa.
BackgroundSpermatogenesis appears to be a relatively well-conserved process even among distantly related animal taxa such as invertebrates and vertebrates. Although Hymenopterans share many characteristics with other organisms, their complex haplodiploid reproduction system is still relatively unknown. However, they serve as a complementary insect model to Drosophila for studying functional male fertility. In this study, we used a comparative method combining taxonomic, phenotypic data and gene expression to identify candidate genes that could play a significant role in spermatogenesis in hymenopterans.ResultsOf the 546 mouse genes predominantly or exclusively expressed in the mouse testes, 36% had at least one ortholog in the fruit fly. Of these genes, 68% had at least one ortholog in one of the six hymenopteran species we examined. Based on their gene expression profiles in fruit fly testes, 71 of these genes were hypothesized to play a marked role in testis function. Forty-three of these 71 genes had an ortholog in at least one of the six hymenopteran species examined, and their enriched GO terms were related to the G2/M transition or to cilium organization, assembly, or movement. Second, of the 379 genes putatively involved in male fertility in Drosophila, 224 had at least one ortholog in each of the six Hymenoptera species. Finally, we showed that 199 of these genes were expressed in early pupal testis in Nasonia vitripennis; 86 exhibited a high level of expression, and 54 displayed modulated expression during meiosis.ConclusionsIn this study combining phylogenetic and experimental approaches, we highlighted genes that may have a major role in gametogenesis in hymenopterans; an essential prerequisite for further research on functional importance of these genes.
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