期刊
STAR PROTOCOLS
卷 2, 期 2, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2021.100487
关键词
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资金
- Swedish Research Council [2015-00174]
- Marie Sklodowska Curie Actions [INCA 600398]
- Jonas Soderquist Foundation
- Petrus and Augusta Hedlund Foundation
- Tsinghua-Berkeley Shenzhen Institute
- Tsinghua Shenzhen International Graduate School
- Singapore National Resarch Foundation [NRF2012NRF-CRP001-056]
- Singapore Ministry of Health [MOE2019-T2-2-130]
- Singapore National Medical Research Council [MOH-000386, OFIRG19NOV-0050]
- Swedish Research Council [2015-00174] Funding Source: Swedish Research Council
Bats carry viruses of global public health significance. Understanding bat immune systems can provide intervention strategies for disease prevention. Studying bat unconventional T cells can help with immunological research in other bat species.
Bats harbor viruses of global public health significance. Understanding bat immune systems may provide intervention strategies to prevent zoonotic disease transmission and identify therapeutic targets. This protocol describes how to culture and expand pteropid bat unconventional T cells, restricted by the MHC-I-related protein 1 (MR1), an MHC-I-like protein. Using multicolor flow-cytometry-based techniques, we examine pteropid MR1T cell functionality, including proliferative capacity, cytotoxicity, and cytokine production. This protocol can be adapted to aid immunological research in other bat species.For complete details on the use and execution of this protocol, please refer to Leeansyah et al. (2020b)
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