期刊
ACS FOOD SCIENCE & TECHNOLOGY
卷 1, 期 5, 页码 908-916出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsfoodscitech.1c00009
关键词
plant-based protein; technical functionality protein concentrates; gelation; protein isolation; novel protein sources; RuBisCO; biorefinery; duckweed
Optimized isolation procedure resulted in increased protein yield and RuBisCO enrichment from duckweed. Duckweed protein properties varied at different pH levels, with stronger gelling observed at pH 7.
An isolation procedure for proteins from duckweed was optimized based on a previously developed method for protein isolation from sugar beet leaves. Optimization included the protocol for disrupting cells and protein recovery. With the optimized protocol, protein was isolated (protein yield 14.2%, RuBisCO yield 27%). The concentrate was off-white and contained 67.2% protein. The isolation procedure resulted in a large enrichment in RuBisCO (from 48% to 92%). Denaturation of duckweed protein concentrate was observed at 62 degrees C at pH 7, while heating at pH 4 did not show denaturation peaks. Solubility was good far from the iso-electric point and showed a minimum around pH 5. Gelling was better at pH 7 than at pH 4. At pH 7, duckweed gels were much stronger than soy and only slightly weaker compared to egg white protein, while at pH 4 duckweed gel strength was similar to soy and lower than egg white.
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