Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing

Distribution of cells and proteins in whole organs, such as the brain, can provide another level of insight into molecular and cellular functions. Here, we describe a whole-brain immunostaining method using the turquoise killifish, an emerging model for aging research. We optimized a protocol for tissue clearing and whole-brain immunostaining to the turquoise killifish brain. This protocol provides a comprehensive procedure from brain dissection to whole-brain imaging and image processing.For complete details on the use and execution of this protocol, please refer to Eunjeong Do (2020) and Lee et al. (2021).

Automated summary

Researchers have optimized a whole-brain immunostaining method using turquoise killifish as a model, providing deeper insights into the distribution of cells and proteins in organs. The protocol offers a comprehensive procedure from brain dissection to imaging and image processing.