Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin

Current knowledge on brain oligodendrogenesis, composed of three sequential waves from different regions, is mainly obtained from rodent studies. Oligoden-droglial development and myelination in the rodent brain may not fully mirror those processes in the human brain. Here, we provide a step-by-step protocol for generating fused forebrain organoids derived from human pluripotent stem cells. These fused organoids recapitulate human oligodendroglial develop-ments, offering innovative insights into human myelination research in vitro.For complete details on the use and execution of this protocol, please refer to Cameron-Curry and Le Douarin (1995), Kessaris et al. (2006), and Kim et al. (2019).

Automated summary

The article provides a step-by-step protocol for generating fused forebrain organoids derived from human pluripotent stem cells, which recapitulate human oligodendroglial development. The protocol offers innovative insights into human myelination research in vitro.