期刊
STAR PROTOCOLS
卷 2, 期 2, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2021.100511
关键词
-
资金
- NIH [RO1 DK11003401, ADA 118-IBS280]
- core services of the Diabetes Research Center and the Flow Cytometry Core at Washington University School of Medicine
The study provides a detailed protocol for analyzing liver macrophage subsets in mice with NAFLD and early NASH using flow cytometry and immunofluorescence. These methods can help assess the composition and localization of macrophage subsets during NASH.
Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we pro-vide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the compo-sition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).
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