Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells

BMI1-expressing cancer stem cells (CSCs) play a key role in the development, progression, therapy resistance, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC). Here, we present a chemically-induced HNSCC mouse model, genetically and pathologically similar to human HNSCC. This protocol describes how to use genetic lineage tracing based on the CreloxP recombination strategy, which allows us to study the regulation and targeting of BMI1+ CSCs in primary tumors and lymph node metastases. For complete details on the use and execution of this protocol, please refer to Chen et al. (2017) and Jia et al. (2020)

Automated summary

Cancer stem cells expressing BMI1 are crucial in the development, progression, therapy resistance, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC). A chemically-induced HNSCC mouse model genetically and pathologically similar to human HNSCC is presented, along with a genetic lineage tracing protocol to study the regulation and targeting of BMI1+ CSCs in primary tumors and lymph node metastases. For detailed information, refer to Chen et al. (2017) and Jia et al. (2020).