期刊
EXPERIMENTAL PHYSIOLOGY
卷 102, 期 12, 页码 1619-1634出版社
WILEY
DOI: 10.1113/EP086589
关键词
peroxisome proliferator activated receptor- coactivator-1 (PGC-1); atria; action potential; cardiac arrhythmia
类别
资金
- Wellcome Trust [105727/Z/14/Z]
- Medical Research Council [MR/M001288/1]
- British Heart Foundation [PG/14/79/31102, PG/15/12/31280]
- Isaac Newton Trust-Wellcome Trust ISSF-University of Cambridge
- Sudden Arrhythmic Death Syndrome (SADS) UK society
- Ministry of Education, Malaysia [FRGS/2014/SKK01/PERDANA/02/1]
- MRC [MR/M001288/1] Funding Source: UKRI
- British Heart Foundation [PG/14/79/31102] Funding Source: researchfish
- Medical Research Council [MR/M001288/1] Funding Source: researchfish
New Findings What is the central question of this study? Can we experimentally replicate atrial pro-arrhythmic phenotypes associated with important chronic clinical conditions, including physical inactivity, obesity, diabetes mellitus and metabolic syndrome, compromising mitochondrial function, and clarify their electrophysiological basis? What is the main finding and its importance? Electrocardiographic and intracellular cardiomyocyte recording at progressively incremented pacing rates demonstrated age-dependent atrial arrhythmic phenotypes in Langendorff-perfused murine Pgc1(-/-) hearts for the first time. We attributed these to compromised action potential conduction and excitation wavefronts, whilst excluding alterations in recovery properties or temporal electrophysiological instabilities, clarifying these pro-arrhythmic changes in chronic metabolic disease. Atrial arrhythmias, most commonly manifesting as atrial fibrillation, represent a major clinical problem. The incidence of atrial fibrillation increases with both age and conditions associated with energetic dysfunction. Atrial arrhythmic phenotypes were compared in young (12-16week) and aged (>52week) wild-type (WT) and peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b)-deficient (Pgc1(-/-)) Langendorff-perfused hearts, previously used to model mitochondrial energetic disorder. Electrophysiological explorations were performed using simultaneous whole-heart ECG and intracellular atrial action potential (AP) recordings. Two stimulation protocols were used: an S1S2 protocol, which imposed extrasystolic stimuli at successively decremented intervals following regular pulse trains; and a regular pacing protocol at successively incremented frequencies. Aged Pgc1(-/-) hearts showed greater atrial arrhythmogenicity, presenting as atrial tachycardia and ectopic activity. Maximal rates of AP depolarization (dV/dt(max)) were reduced in Pgc1(-/-) hearts. Action potential latencies were increased by the Pgc1(-/-) genotype, with an added interactive effect of age. In contrast, AP durations to 90% recovery (APD(90)) were shorter in Pgc1(-/-) hearts despite similar atrial effective recovery periods amongst the different groups. These findings accompanied paradoxical decreases in the incidence and duration of alternans in the aged and Pgc1(-/-) hearts. Limiting slopes of restitution curves of APD(90) against diastolic interval were correspondingly reduced interactively by Pgc1(-/-) genotype and age. In contrast, reduced AP wavelengths were associated with Pgc1(-/-) genotype, both independently and interacting with age, through the basic cycle lengths explored, with the aged Pgc1(-/-) hearts showing the shortest wavelengths. These findings thus implicate AP wavelength in possible mechanisms for the atrial arrhythmic changes reported here.
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