期刊
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
卷 57, 期 3, 页码 351-355出版社
PLEIADES PUBLISHING INC
DOI: 10.1134/S0003683821030169
关键词
hydrogenase; hydrogen; HD isotope exchange; NiFe active center; FeS clusters; enzyme reconstruction
资金
- Russian Science Foundation [19-14-00255]
- Russian Science Foundation [19-14-00255] Funding Source: Russian Science Foundation
The catalytic center of Thiocapsa roseopersicina remains active after treatment with cyanide, and can be restored under certain conditions. The reconstruction of activity depends on time, and substituting isotopes can reduce the required amount.
It is shown that the catalytic center of Thiocapsa roseopersicina remains active after prolonged treatment with cyanide. It was found that the incubation of cyanide-treated hydrogenase in the presence of beta-mercaptoethanol, ferric iron, and sodium sulfide restored the hydrogenase activity in the hydrogen-oxidation reaction in the presence of methylviologen. The process of activity reconstruction depended on time and reached its maximum value (similar to 60%) within 30 min at room temperature. In this case, an absorption band at 420 nm appeared in the absorption spectrum of the hydrogenase, which was present in the native hydrogenase and disappeared after treatment with cyanide; this indicated the reconstruction of iron-sulfur clusters. Thus, instead of growing bacteria in the presence of an iron isotope, one can replace Fe-56 with Fe-57 in the isolated enzyme, which will allow the use of smaller amounts of Fe-57.
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