The robust NMR toolbox for metabolomics

Here, we implemented and validated a suite of selective and non-selective CPMG-filtered 1D and 2D TOCSY/HSQC experiments for metabolomics research. They facilitated the unambiguous identification of metabolites embedded in broad lipid and protein signals. The 2D spectra improved non-targeted analysis by removing the background broad signals of macromolecules.

Automated summary

In this study, a set of selective and non-selective CPMG-filtered 1D and 2D TOCSY/HSQC experiments were implemented and validated for metabolomics research. These experiments aided in the clear identification of metabolites within complex lipid and protein signals and improved non-targeted analysis by removing background signals in 2D spectra.