4.6 Article

Development of a lateral-flow ICA strip for the detection of colchicine

期刊

ANALYTICAL METHODS
卷 13, 期 27, 页码 3092-3100

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ay00804h

关键词

-

资金

  1. National Key Research and Development Program of China [2019YFC1605004]

向作者/读者索取更多资源

This study developed a highly sensitive monoclonal antibody for colchicine detection, and prepared a lateral-flow immunochromatographic assay strip for on-site detection of colchicine in food samples.
Colchicine (COL), which is extracted from colchicine and papaya, is widely used in medicine. However, COL poisoning causes serious adverse complications including death. Therefore, there is a need to develop a sensitive COL detection method. In this study, we developed a highly sensitive monoclonal antibody 1E4 with a half-maximal inhibitory concentration and linear range of 0.43 ng mL(-1) and 0.09-2.16 ng mL(-1), respectively. Using 1E4, we developed a lateral-flow immunochromatographic assay (ICA) strip for COL detection. Based on the results, the detection interval was 1-25 ng mL(-1) in milk, 2.5-50 ng mL(-1) in beef, 1-25 ng mL(-1) in edible lily and 2.5-25 ng mL(-1) in daylily. The lateral-flow ICA strip can be used as an effective tool for COL detection in food samples on site.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据