期刊
ANALYTICAL METHODS
卷 13, 期 27, 页码 3092-3100出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ay00804h
关键词
-
资金
- National Key Research and Development Program of China [2019YFC1605004]
This study developed a highly sensitive monoclonal antibody for colchicine detection, and prepared a lateral-flow immunochromatographic assay strip for on-site detection of colchicine in food samples.
Colchicine (COL), which is extracted from colchicine and papaya, is widely used in medicine. However, COL poisoning causes serious adverse complications including death. Therefore, there is a need to develop a sensitive COL detection method. In this study, we developed a highly sensitive monoclonal antibody 1E4 with a half-maximal inhibitory concentration and linear range of 0.43 ng mL(-1) and 0.09-2.16 ng mL(-1), respectively. Using 1E4, we developed a lateral-flow immunochromatographic assay (ICA) strip for COL detection. Based on the results, the detection interval was 1-25 ng mL(-1) in milk, 2.5-50 ng mL(-1) in beef, 1-25 ng mL(-1) in edible lily and 2.5-25 ng mL(-1) in daylily. The lateral-flow ICA strip can be used as an effective tool for COL detection in food samples on site.
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