Chemical profile of Tetraclinis articulata (Vahl) Masters, and Juglans regia L. and Olea europaea L. var. Sylvestris used against oral diseases: in vitro analysis between polyphenolic content and aqueous extraction optimization

Objective: Optimization of aqueous extraction to extract the maximum amount of polyphenolic compounds that are used to treat oral disorders. Methods: Using revelation tests for phytochemical screening, Folin-Ciocalteu reagent for total phenols, catechin standard for total flavonoids, acidified vanillin for total condensed tannins, and PCA analysis to detect different correlations between plants and between employed extractions. Results: The highest (p < 0.0001); total flavonoides (195,80 +/- 2,91 mg CE/g d.e) was obtained from decocted extract of Olea europaea L. subsp. europaea var. sylvestris, total phenolic (167,71 +/- 12,52 mg GAE/g d.e) and total condensed tannins (250,44 +/- 10,18 mg CE/g d.e) was obtained respectively from soxhlet extract and infused extract of Tetraclinis articulata L. whereas; The correlation analysis using Principal Component Analysis (PCA) was positive between infusion and decoction, between total flavonoids and total phenols which is not correlated with total condensed tannins. Conclusion: Juglans regia L. bark contain a higher level of polyphenolic constituents than leaves, Decoction extraction of Olea europaea L. var. sylvestris leaf recommended to increase the yield of polychenolic extracts, condensed tannins of Tetraclinis articulata L. are degradable in high temperature.

Automated summary

The study aimed to optimize aqueous extraction for treating oral disorders by maximizing polyphenolic compound extraction. Results showed differences in extracted compounds and correlations between different plant parts and extraction methods, with decoction extraction from Olea europaea L. var. sylvestris leaves recommended for increased polyphenolic yield, and the degradability of Tetraclinis articulata L. condensed tannins at high temperatures.