期刊
EXPERIMENTAL AND MOLECULAR PATHOLOGY
卷 103, 期 1, 页码 33-37出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.yexmp.2017.06.007
关键词
Chronic myeloid leukemia; Acute lymphoblastic leukemia; ABL1 mutation; MinION; Next generation sequencing
类别
资金
- Associazione Italiana contro le Leucemie (AIL)-BARI
We report a third-generation sequencing assay on nanopore technology (MinION) for detecting BCR-ABL1 KD mutations and compare the results to a Sanger sequencing(SS)-based test in 24 Philadelphia-positive (Ph +) leukemia cases. Our data indicates that MinION is markedly superior to SS in terms of sensitivity, costs and timesaving, and has the added advantage of determining the clonal configuration of multiple mutations. We demonstrate that MinION is suitable for employment in the hematology laboratory for detecting BCR-ABL1 KD mutation in Ph + leukemias.
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