期刊
PAKISTAN JOURNAL OF ZOOLOGY
卷 53, 期 4, 页码 1233-1242出版社
ZOOLOGICAL SOC PAKISTAN
DOI: 10.17582/journal.pjz/20191202121250
关键词
Buffalo; CSN1S1; Polymorphisms; Variant; Caseins
类别
资金
- National Natural Science Foundation of China [31760659, 31460582]
- Natural Science Foundation Key Project of Yunnan Province, China [2014FA032]
This study identified the polymorphisms in the coding sequences of the CSN1S1 gene in river and swamp buffalo, showing variations that may affect the function of alpha(S1)-CN and result in protein variants specific to each buffalo type. The findings contribute to a better understanding of genetic characteristics in buffalo and highlight differences in protein variants between river and swamp buffalo.
Alpha S1-casein (alpha(S1)-CN) is a major casein in milk, which exerts a crucial role in casein transport and is related to individual milk components, nutritive value and production traits of milk. So far, alpha(S1)-CN coding gene (CSN1S1) has been widely studied in dairy cattle, but the polymorphisms of the CSN1S1 gene have not been fully understood in buffalo. In this study, the polymorphisms in coding sequence (CDS) of the CSN1S1 gene for river and swamp buffalo were detected using PCR product direct sequencing. The CDS for both types of buffalo was the same in length, which contained 645 nucleotides and encoded a peptide composing of 214 residues. A total of 5 single nucleotide polymorphisms (SNPs) was identified in two types of buffalo. Among them, c.516T>C and c.578C>T were observed only in river buffalo, while c.175A>G, c.580T>C and c.609T>G were found only in swamp buffalo. The c.175A>G, c.578C>T and c.580T>C were non-synonymous, which led to substitutions of p.I44V, p.L178S and p.F179L. The prediction showed that the p.F179L may affect the function of buffalo alpha(S1)-CN. Eight buffalo CSN1S1 haplotypes were defined in this study, and accordingly, 6 protein variants and 2 synonymous variants of alpha(S1)-CN were inferred and named. The variants A, B', B '', C, E and F were observed only in river buffalo, whereas variant D was found only in swamp buffalo. The variant B was shared by both types of buffalo with high frequencies. The buffalo variants determined here did not exist in Bos genus. In addition, 9 amino acid differential sites of alpha(S1)-CN between buffalo and Bos genus were identified, of which p.42T and p.115S were located at phosphorylation sites, which may lead to differences in the physicochemical properties of alpha(S1)-CN between buffalo and Bos genus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据