期刊
BMC GENOMIC DATA
卷 22, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12863-021-00975-2
关键词
DNA methylation; Quantitative trait loci; Cardiac tissue; Bayesian co-localization; Mendelian randomization
资金
- National Heart, Lung and Blood Institute [K01HL140333]
- Eunice Kennedy Shriver National Institute of Child Health and Human Development [R03HD092854, R01HD039054]
- National Institute of Dental and Craniofacial Research [R03DE024198, R03DE025646]
The study found that genetic variants may influence the risk of CHDs by regulating changes in DNA methylation and gene expression, providing new insights into the pathogenesis of CHDs. The results can be integrated with other genetic studies of heart diseases, especially CHDs, and are significant for the prevention and treatment of CHDs.
Background Most congenital heart defects (CHDs) result from complex interactions among genetic susceptibilities, epigenetic modifications, and maternal environmental exposures. Characterizing the complex relationship between genetic, epigenetic, and transcriptomic variation will enhance our understanding of pathogenesis in this important type of congenital disorder. We investigated cis-acting effects of genetic single nucleotide polymorphisms (SNPs) on local DNA methylation patterns within 83 cardiac tissue samples and prioritized their contributions to CHD risk by leveraging results of CHD genome-wide association studies (GWAS) and their effects on cardiac gene expression. Results We identified 13,901 potential methylation quantitative trait loci (mQTLs) with a false discovery threshold of 5%. Further co-localization analyses and Mendelian randomization indicated that genetic variants near the HLA-DRB6 gene on chromosome 6 may contribute to CHD risk by regulating the methylation status of nearby CpG sites. Additional SNPs in genomic regions on chromosome 10 (TNKS2-AS1 gene) and chromosome 14 (LINC01629 gene) may simultaneously influence epigenetic and transcriptomic variations within cardiac tissues. Conclusions Our results support the hypothesis that genetic variants may influence the risk of CHDs through regulating the changes of DNA methylation and gene expression. Our results can serve as an important source of information that can be integrated with other genetic studies of heart diseases, especially CHDs.
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