期刊
RSC CHEMICAL BIOLOGY
卷 2, 期 5, 页码 1370-1383出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cb00207k
关键词
-
资金
- Simons Foundation Collaboration on the Origins of Life [290356FY18]
- NIH New Innovator Program [DP2GM123457]
- NSF [1935087]
- NSF-CBET [1804220]
- NASA [20-EXO20-0110]
- Direct For Biological Sciences
- Emerging Frontiers [1935087] Funding Source: National Science Foundation
- Directorate For Engineering [1804220] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys [1804220] Funding Source: National Science Foundation
Various self-cleaving ribozymes found in nature can catalyze the sequence-specific intramolecular cleavage of RNA and be engineered to cleave appropriate substrates in an intermolecular fashion. While these ribozymes share a common catalytic mechanism, each family exhibits unique overall architecture and active site organization.
Various self-cleaving ribozymes appearing in nature catalyze the sequence-specific intramolecular cleavage of RNA and can be engineered to catalyze cleavage of appropriate substrates in an intermolecular fashion, thus acting as true catalysts. The mechanisms of the small, self-cleaving ribozymes have been extensively studied and reviewed previously. Self-cleaving ribozymes can possess high catalytic activity and high substrate specificity; however, substrate specificity is also engineerable within the constraints of the ribozyme structure. While these ribozymes share a common fundamental catalytic mechanism, each ribozyme family has a unique overall architecture and active site organization, indicating that several distinct structures yield this chemical activity. The multitude of catalytic structures, combined with some flexibility in substrate specificity within each family, suggests that such catalytic RNAs, taken together, could access a wide variety of substrates. Here, we give an overview of 10 classes of self-cleaving ribozymes and capture what is understood about their substrate specificity and synthetic applications. Evolution of these ribozymes in an RNA world might be characterized by the emergence of a new ribozyme family followed by rapid adaptation or diversification for specific substrates.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据