期刊
LAB ON A CHIP
卷 21, 期 15, 页码 2892-2900出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1lc00421b
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资金
- Science and Technology Innovation Team Project of Foshan, P.R.China [1920001000047]
- Key R&D Project of Science and Technology Development Plan of Jilin Province, P.R.China [20200404148YY]
- 111 Project of China [D17017]
- Developing Project of Science and Technology of Jilin Province [202002040JC, 20200201266JC, 20200201271JC, 20190201181JC]
- International Science and Technology Cooperation Project of Jilin Province [20190701029GH]
A one-to-three droplet splitting technique was proposed in this study to generate micro-droplets in digital microfluidics for parallel detection, allowing for the retention of magnetic beads by reduced magnetic force. The technique was demonstrated in an on-chip magnetic bead chemiluminescence immunoassay, showing promising results for in vitro diagnostic applications.
In digital microfluidics, droplet generation is a fundamental operation for quantitative liquid manipulation. The generation of well-defined micro-droplets on a chip with restricted device geometries has become a real obstacle for digital microfluidics platforms to be used in parallel for in vitro diagnostic applications. Here, we propose a one-to-three droplet splitting technique that is able to generate sub-microlitre droplets beyond the well-known geometry limit in electrowetting-on-dielectric digital microfluidics. Accordingly, we realized an on-chip magnetic bead chemiluminescence immunoassay for parallel detection with the one-to-three technique. With the help of the generated micro droplets, we were able to retain the magnetic beads by a significantly reduced magnetic force. We have shown the detection of five B-type natriuretic peptide analyte samples on a single chip for around 10 minutes. The correlation coefficient of the calibration curve was 0.9942, and the detection limit was lower than 5 pg mL(-1).
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