期刊
ACS APPLIED BIO MATERIALS
卷 4, 期 6, 页码 4849-4861出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.1c00133
关键词
cardiogel; polypyrrole; conductive scaffold; cardiac progenitor cell; myocardial infarction
资金
- Royan Institute
The study successfully improved cardiac function and CPC retention in rat hearts by transplanting CPC using a PPy-doped scaffold, demonstrating its therapeutic potential.
Myocardial infarction (MI) irreversibly injures the heart tissue. Cardiovascular tissue engineering has been developed as a promising therapeutic approach for post-MI repair. Previously, we discovered the ability of a polypyrrole (PPO-incorporated cardiogel (CG) for improvement of maturity and functional synchrony of rat neonatal cardiomyocytes. Here, we used the cross-linked form of PPy-incorporated CG (CG-PPy), in order to improve electromechanical properties of scaffold, for application in cardiac progenitor cell (CPC) transplantation on post-MI rat hearts. Improved mechanical property and electrical conductivity (sixfold) were evident in the cross-linked CG-PPy (P1) compared to cross-linked CG (C1) scaffolds. Transplantation of CPC-loaded P1 (P1-CPC) resulted in substantial improvement of cardiac functional properties. Furthermore, lower fibrotic tissue and higher CPC retention were observed. The grafted cells showed cardiomyocyte characteristics when stained with human cardiac troponin T and connexin43 antibodies, while neovessel formation was similarly prominent. These findings highlight the therapeutic promise of the P1 scaffold as a CPC carrier for functional restoration of the heart post-MI.
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