Interparticle effects in the cotransport of viruses and engineered nanoparticles in saturated porous media

The effects of P25 TiO2 nanoparticles on the transport in saturated porous media of the bacteriophage PP7 was investigated. Two levels of ionic strength were considered, given by monovalent and divalent cations, Na+ and Ca2+. The viruses and the P25 particles were characterized with respect to size and surface charge, by dynamic light scattering and electron microscopy. The breakthrough curves were obtained for suspensions of each particle and both combined, using UV absorbance to quantify P25 and RT-qPCR for PP7. In the single transport experiments, P25 was retained, while the viruses mostly eluted the sand bed. However, in the cotransport experiments, retention increased for the virus and decreased for the TiO2 nanoparticles under high viral content, due to heteroaggregation among the colloids in the incoming suspension, electrostatic interactions, and ripening. The interparticle interactions were modeled by DLVO theory, resulting in agreement with the experimental observations, which highlights the electrostatic nature.

Automated summary

The study investigated the effects of P25 TiO2 nanoparticles on the transport of bacteriophage PP7 in saturated porous media. It was found that in co-transport experiments, retention of the virus increased and retention of TiO2 nanoparticles decreased under high viral content due to heteroaggregation among colloids, electrostatic interactions, and ripening. The interparticle interactions were successfully modeled by DLVO theory, highlighting the electrostatic nature of the interactions.