Chemo-enzymatic synthesis of Lacto-N-biose I catalyzed by β-1,3-galactosidase from Bacillus circulans using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside as a glycosyl donor

Chemo-enzymatic synthesis of lacto-N-biose I (LNB) catalyzed by beta-1,3-galactosidase from Bacillus circulans (BgaC) has been developed using 4,6-dimethoxy-1,3,5-triazin-2-yl beta-galactopyranoside (DMT-beta-Gal) and GlcNAc as the donor and acceptor substrates, respectively. BgaC transferred the Gal moiety to the acceptor, giving rise to LNB. The maximum yield of LNB was obtained at the acceptor : donor substrate ratio of 1:30.

Automated summary

The chemo-enzymatic synthesis of lacto-N-biose I (LNB) catalyzed by beta-1,3-galactosidase from Bacillus circulans (BgaC) was achieved using 4,6-dimethoxy-1,3,5-triazin-2-yl beta-galactopyranoside (DMT-beta-Gal) and GlcNAc as substrates. BgaC transferred the Gal moiety to the acceptor to produce LNB, with the maximum yield obtained at a ratio of 1:30 for acceptor to donor substrates.