期刊
FEMS YEAST RESEARCH
卷 21, 期 5, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsyr/foab035
关键词
anaerobic; cultivation; oxygen requirements; saccharomyces; nonconventional yeast
资金
- European Research Council [694633]
- European Research Council (ERC) [694633] Funding Source: European Research Council (ERC)
This paper discusses critical points in the design of anaerobic yeast cultivation experiments, including serial transfer or continuous cultivation, systematic inclusion of control strains, and minimizing the impact of oxygen diffusion. Basic protocols for anaerobic-chamber and bioreactor experiments are also presented.
All known facultatively fermentative yeasts require molecular oxygen for growth. Only in a small number of yeast species, these requirements can be circumvented by supplementation of known anaerobic growth factors such as nicotinate, sterols and unsaturated fatty acids. Biosynthetic oxygen requirements of yeasts are typically small and, unless extensive precautions are taken to minimize inadvertent entry of trace amounts of oxygen, easily go unnoticed in small-scale laboratory cultivation systems. This paper discusses critical points in the design of anaerobic yeast cultivation experiments in anaerobic chambers and laboratory bioreactors. Serial transfer or continuous cultivation to dilute growth factors present in anaerobically pre-grown inocula, systematic inclusion of control strains and minimizing the impact of oxygen diffusion through tubing are identified as key elements in experimental design. Basic protocols are presented for anaerobic-chamber and bioreactor experiments.
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