期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 85, 期 6, 页码 1536-1545出版社
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbab065
关键词
lactic acid bacteria; membrane vesicle; gut immunity; IgA; Peyer's patches
类别
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [16K18302, 18K04857, 17K06935, 15H05790, 16H01373, 17H04134, 26293111]
- Kansai University
- Japan Agency for Medical Research and Development [JP17fk0108223h0002, JP17ek0410032s0102, JP17fk0108207h0002, JP17ek0210078h0002, JP17ak0101068h0001, JP17gm1010006s0101, JP20gm1010006h0004, JP20ak0101068h0004]
- Ministry of Health, Labour, and Welfare of Japan
- Terumo Foundation for Life Sciences and Arts
- ONO Medical Research Foundation
- Canon Foundation
- Public/Private R&D Investment Strategic Expansion PrograM: PRISM
- Grants-in-Aid for Scientific Research [16K18302, 18K04857, 17K06935, 17H04134] Funding Source: KAKEN
Utilizing membrane vesicles derived from Lactobacillus sakei subsp enhances IgA production in Peyer's patch cells, while inducing nitric oxide and interleukin-6 via Toll-like receptor 2 on bone marrow-derived dendritic cells. Inhibition of inducible nitric oxide synthase and retinaldehyde dehydrogenase 2, as well as neutralization of IL-6 can suppress the enhanced IgA production elicited by membrane vesicle stimulation.
We analyzed the mechanisms underlying enhanced IgA production in the cells of Peyer's patch cells via membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC 15893. Depletion of CD11c(+) cells from Peyer's patch cells suppressed the enhanced IgA production mediated by membrane vesicles. Meanwhile, the stimulation of bone-marrow-derived dendritic cells with membrane vesicles increased gene expression of inducible nitric oxide synthase, retinaldehyde dehydrogenase 2, and several inflammatory cytokines. The production of nitric oxide and interleukin (IL)-6 by membrane vesicle stimulation was induced via Toll-like receptor 2 on bone marrow-derived dendritic cells. Inhibition of inducible nitric oxide synthase and retinaldehyde dehydrogenase 2, as well as the neutralization of IL-6 in Peyer's patch cells, suppressed the enhanced IgA production by membrane vesicle stimulation. Hence, nitric oxide, retinoic acid, and IL-6 induced by membrane vesicles play crucial roles in the enhanced IgA production elicited by membrane vesicles in Peyer's patch cells.
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