Synthesis of Tin Oxide Nanoparticles by Pulsed Laser Ablation Method Using Low-Energy Nd: YAG Laser as an Antibacterial Agent

Tin oxide nanoparticles (SnNPs) are very useful to be employed as an antibacterial agent for both gram-positive and gram-negative bacteria. In this present work, the synthesis of SnNPs was successfully carried out using the neodymium yttrium aluminum garnet (Nd:YAG) laser with a wavelength of 1064 nm, pulse duration of 7 ns, and a laser frequency of 10 Hz. Experimentally, a pulse Nd:YAG laser was directed and focused on a high-purity tin (Sn) metal, immersed in various liquid media including pure water and ethylene glycol. A brownish colloidal colour was produced both in pure water and ethylene glycol liquid media. Characterizations of tin oxide nanoparticles were made using UV-Vis, EDX, FTIR, and TEM. UV-Vis characterization produced absorbance values in pure water and ethylene glycol media of 1.314 a.u. and 1.119 a.u., respectively. TEM images show that the shape of tin oxide nanoparticles produced is spherical. Measurement of nanoparticle size distribution was made using image-J software and the average diameter of nanosize in the ethylene glycol medium is 12.55 nm, which is smaller than the size in the pure water of 19.98 nm. The EDX spectrum analysis results show that there are only Sn and 0 atoms in colloidal tin oxide nanoparticles (SnNPs). FTIR results show the formation of tin oxide (SnO2) spectrum at the wavenumber of 629.03 cm(-1). The produced colloidal SnNPs were then applied as an antibacterial agent of E. coli using the disk diffusion method. Results certified that various concentrations of SnNPs of 10 ppm, 20 ppm, and 30 ppm gain the diameter of inhibition zone (DIZ) in sequence 6.50 mm, 6.75 mm, and 9.50 mm. Based on these experimental results, it shows that the higher the concentration of SnNPs given, the greater the ability to degrade and inhibit bacteria.

Automated summary

Tin oxide nanoparticles (SnNPs) synthesized using Nd:YAG laser demonstrate potential as an antibacterial agent, with higher concentrations showing greater efficacy in degrading and inhibiting bacteria.