期刊
NANOSCALE
卷 13, 期 29, 页码 12687-12696出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1nr01970h
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资金
- China Scholarship Council-University of Oxford Scholarship
- ERC Consolidator Grant [819593]
- EPSRC Leadership Fellowship [EP/T03419X/1]
- Dutch Organisation for Scientific Research (NWO) [16PR3238]
- EPSRC [EP/T03419X/1] Funding Source: UKRI
- European Research Council (ERC) [819593] Funding Source: European Research Council (ERC)
This study presents a molecular force sensor and charge detector based on tracking tethered double-stranded DNA functionalised with charged nanoparticles. The approach allows for real-time detection and quantification of electrophoretic force and changes in particle charge state down to the sub-piconewton scale, providing an alternative route for studying structural and charge dynamics at the single molecule level.
Measuring the electrophoretic mobility of molecules is a powerful experimental approach for investigating biomolecular processes. A frequent challenge in the context of single-particle measurements is throughput, limiting the obtainable statistics. Here, we present a molecular force sensor and charge detector based on parallelised imaging and tracking of tethered double-stranded DNA functionalised with charged nanoparticles interacting with an externally applied electric field. Tracking the position of the tethered particle with simultaneous nanometre precision and microsecond temporal resolution allows us to detect and quantify the electrophoretic force down to the sub-piconewton scale. Furthermore, we demonstrate that this approach is suitable for detecting changes to the particle charge state, as induced by the addition of charged biomolecules or changes to pH. Our approach provides an alternative route to studying structural and charge dynamics at the single molecule level.
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