4.8 Article

A BLUS1 kinase signal and a decrease in intercellular CO2 concentration are necessary for stomatal opening in response to blue light

期刊

PLANT CELL
卷 33, 期 5, 页码 1813-1827

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OXFORD UNIV PRESS INC
DOI: 10.1093/plcell/koab067

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资金

  1. JSPS KAKENHI [18H02468, 26711019, 15K14552, 20H05420]
  2. Japan Foundation for Applied Enzymology
  3. Cooperative Research Project Program of the Medical Institute of Bioregulation, Kyushu University
  4. Grants-in-Aid for Scientific Research [20H05420, 18H02468, 15K14552, 26711019] Funding Source: KAKEN

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The study shows that blue light-induced stomatal opening in plants is mediated by the BLUS1 signaling pathway, which regulates the opening and closure of stomata, thereby affecting CO2 uptake and transpiration.
Light-induced stomatal opening stimulates CO2 uptake and transpiration in plants. Weak blue light under strong red light effectively induces stomatal opening. Blue light-dependent stomatal opening initiates light perception by phototropins, and the signal is transmitted to a plasma membrane H+-ATPase in guard cells via BLUE LIGHT SIGNALING 1 (BLUS1) kinase. However, it is unclear how BLUS1 transmits the signal to H+-ATPase. Here, we characterized BLUS1 signaling in Arabidopsis thaliana, and showed that the BLUS1 C-terminus acts as an auto-inhibitory domain and that phototropin-mediated Ser-348 phosphorylation within the domain removes auto-inhibition. C-Terminal truncation and phospho-mimic Ser-348 mutation caused H+-ATPase activation in the dark, but did not elicit stomatal opening. Unexpectedly, the plants exhibited stomatal opening under strong red light and stomatal closure under weak blue light. A decrease in intercellular CO2 concentration via red light-driven photosynthesis together with H+-ATPase activation caused stomatal opening. Furthermore, phototropins caused H+-ATPase dephosphorylation in guard cells expressing constitutive signaling variants of BLUS1 in response to blue light, possibly for fine-tuning stomatal opening. Overall, our findings provide mechanistic insights into the blue light regulation of stomatal opening.

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